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Transcriptomics
Pierre Frasse, Mohamed Zouine, Mondher Bouzayen

Functional genomic studies are dealing with the characterization of the regulation pathway by which genes interact during biological process.

Microarrays technology has become a widespread tool in functional genomics. They allow the measurement of the expression of thousand of genes simultaneously in order to get better view of the biological systems.

This tool is implemented in all the projects developed in our laboratory and help to the definition of target genes involved in the regulation of the different pathways with regard to biotic or abiotic stress.

Our laboratory elaborate the tomato microarrays called : EU Tom1 12K oligo-arrays.

They contain 12,160 long oligos (70 mers) including 300 controls randomly placed throughout the array.

11,860 genes from the tomato are represented, the majority of the probes being designed from gene sequences gathered from the Lycopersicon Combined Build #3 Unigene database at Cornell University. The remaining number of oligos was designed from sequences in GenBank.

For complete documentations on the array see below:

  • EU_TOM1_12K description
  • GenePix .Gal file for EU_TOM1_12K microarrays
  • Gene ID file for EU_TOM1_12K microarrays
  • Protocol for target synthesis (labelled aRNA)
  • Protocol for manual hybridization


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Laboratoire de Genomique et Biotechnologie des Fruits, UMR 990 INRA/INP-ENSAT
Chemin de borderouge BP107, 31326 Castanet Tolosan Cedex
tel: +33 5 62 19 35 79, fax: +33 5 62 19 35 73


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